Instructions for DNA Dog Testing

1. Complete the Customer Information Card.

2. Do not feed the dog for at least 1 hour before sampling. Allow the dog to drink plenty of water. Check the dog’s mouth to ensure no food is present.

3. If sampling multiple dogs, wash hands between each animal sampling to reduce the chance of DNA contamination.

NOTE: The DNA Dog Test is NOT a saliva test. Cheek cells from the inside of your dog’s mouth are required for the DNA Test to work correctly.

4. Remove the foam-tipped swab A, DNA ID card and the polyester tipped applicator (swab B) (see figure 1). To insure that we receive adequate Dog DNA cells for analysis, we ask that you use both swabs for Dog DNA collection.

5. Place the foam end of the swab A into the dog’s mouth and rub the pad in a circular motion against the inside of the cheek for several seconds, rotating the pad so that each side of the pad is used. Swab inside each cheek twice for a total of FOUR swabbings. This will improve your chances on obtaining your Dog's DNA first time around.

6. Place the DNA ID card onto a firm surface and place the foam end of the swab A onto the pink portion of the card in the center of the circle (see figure 3). Roll one side of the foam swab back and forth to transfer the dog’s cheek cells onto the card. Fold the paper flap over to cover the foam swab. Press firmly for ten seconds. Open flap, turn the swab over, place back on the DNA ID card. Repeat rolling and pressing process.

7. Remove the swab A from the DNA card and inspect the DNA ID card. For successful DNA collection, at least 50% of the circle on the DNA ID card will have changed from the original dark pink color to a lighter shade of pink to white.

8. If no area of the circle has changed color, repeat steps 5 through 7.

9. Some breeds of dogs have a dry mouth. If the DNA ID Card has not changed color after two attempts, add between 3 and 6 drops of water to the foam pad and repeat steps 5 through 7.

10. Once the color change has been observed, allow the DNA ID card to dry with the flap open for 30 minutes at room temperature.

11. The polyester tipped applicator (swab B) is a stand alone device, and does not need to be applied to a DNA ID Card. Simply collect the Dog DNA cells using the same swabbing technique and place the swab in the DNA Dog Test's prepaid envelope.

12. Write your dog’s name on the card and then place the card, both swabs and the completed Customer Information Card directly into the enclosed prepaid envelope and mail to BioPet Vet lab.

13. Your dog’s Ancestry Analysis Certificate and Breed Behavior, Health and Personality Summary will be mailed to you along with the DNA ID Card which can be used as a permanent identification tool for your dog.

Breed Identification Chart

AAfghan Hound AiredaleTerrier Akita Australian Shepherd	BBasenji Basset Hound Beagle Belgian Tervuren Bernese Mountain Dog Bichon Frise Border Collie Borzoi Boston Terrier Boxer Brittany Bulldog Bull Terrier	CChihuahua Chinese Shar-Pei Chow Chow Cocker Spaniel Collie	DDachshund Dalmatian Doberman Pinscher
EEnglish Setter English Springer Spaniel	GGerman Shepherd German Shorthaired Pointer Golden Retriever Great Dane Great Pyrenees Greyhound	IIrish Setter Italian Greyhound	KKeeshond
LLabrador Retriever Lhasa Apso	MMaltese Mastiff Miniature Pinscher	NNewfoundland Norwegian Elkhound	PPapillon Parson Russell Terrier Pekingese Pembroke Welsh Corgi Pomeranian Poodle Pug
RRhodesian Ridgeback Rottweiler	SSaint Bernard Saluki Schnauzer Scottish Terrier Shetland Sheepdog ShihTzu Siberian Husky	WWeimaraner West Highland White Terrier	YYorkshire Terrier